張文眾  李寧  李蓉¹

 中國疾病預防控制中心營(yíng)養與食品安全所，北京，100050

 

摘要：目的探索大豆異黃酮經(jīng)口攝入內分泌干擾毒性，以及劑量-反應關(guān)系；通過(guò)和染料木素比較探索大豆異黃酮雌激素樣作用的主要活性成分。方法 采用子宮增重試驗，分別經(jīng)口灌胃給與0.1、 0.2 、0.5 和1.5 g/kg的大豆異黃酮和0.05、0.10、0.25、和0.75 g/kg染料木素，以及3.0µg/kg 的雌二醇、水和油對照，連續3天。實(shí)驗結束稱(chēng)量子宮的濕重和干重，對卵巢、子宮和陰道進(jìn)行組織病理學(xué)檢查，并且對相應劑量?jì)煞N受試物的效應進(jìn)行比較。結果 大豆異黃酮和染料木素均誘導子宮重量明顯增加，子宮和陰道的組織病理學(xué)明顯增生，并有劑量-反應關(guān)系。染料木素組和含有等量染料木素的大豆異黃酮組的雌激素作用無(wú)差異。結論 在子宮增重試驗中，染料木素是大豆異黃酮的主要活性成分；經(jīng)口攝入SIF對未成年青春期前的雌性大鼠產(chǎn)生內分泌干擾毒性，并有劑量-反應關(guān)系。

關(guān)鍵詞：子宮增重試驗 大豆異黃酮 染料木素 雌激素作用

中圖分類(lèi)號：                            文獻標識碼：

ZHANG Wenzhong, LI Ning, LI Rong

Institute of Nutrition and Food Safety , Chinese Center for Disease Prevention and Control, Beijing, 100050, China

 

Abstract: Object The study aimed to investigate the endocrine disruptive toxicity of isoflavone in vivo, through comparing with genistein, to study main active parts of isoflavone in estrogenic active, also. Methods Uterotrophic assay was carried out. 0.1, 0.2, 0.5, 1.5g/kg bw isoflavone, 0.05, 0.10, 0.25, 7.50g/kg bw genistein, 3.0µg/kg estradiol as positive control, water and oil as control were respectively administered orally to the animal for 3 consecutive day. In the end of the test, uterine wet and blotted weight, histopathology of ovary, uterus and vaginal were measured. Finally, all the effects caused by isoflavone and geinistein were compared.Results Both isoflavone and genistein induce uterine weight increase significantly; histopathology of uterus and vagina showed that both isoflavone and genistein induce proliferation; there are dose-response relationship; in the related dose level, isoflavone and genistein caused similar effects. Conclusion In uterotrophic assay, genistein was the main active part of isoflavone; via oral administration to immature prepuberty rat, isoflavone had endocrine disruptive effects which had obviously dose-response relationship.

Key words: uterotrophic, isoflavone, genistein, estrogenicity, rat

 

 

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